The Division of Pulmonary Medicine in the Department of Pediatrics is seeking a full-time Research Assistant to join a productive laboratory to assist with research on the cell biology of pulmonary fibrosis and genetic lung diseases in adults and children.
The position will require performance of a combination of molecular and cell biology as well as designated work in maintaining and performing experiments using transgenic mouse colonies.
Under moderate supervision the Research Assistant II will:
Understand and perform routine procedures including mouse colony management and standard PCR.
Perform real time PCR, western blotting, ELISA, immunostaining, and cell culture.
Become proficient with tissue dissection/animal injections.
Become proficient in proper processing and catologing of human samples for genetic, genomic, and proteomic research
Assist in other laboratory and research functions.
Key Functions and Expected Performances:
1.Under moderate supervision:
Understand and follow experimental protocols, including animal handling, cell isolation and tissue harvest, cell culture, and molecular assays
Set up, conduct experiments, and continue research projects following protocols
Prepare and operate equipment in experimental procedures and maintain laboratory inventory and equipment
Follow University safety protocols, and laboratory protocols including maintaining sterile conditions as required
Maintain inventories and process and catalog specimens
Perform accurate calculations in the preparation of reagents and solutions
Keep detailed documentation of all procedures and protocols in a lab notebook and electronically as appropriate
Compile and communicate results to PI and lab manager, including the appropriate recording of data and reporting of standard or variant results
Recognize and communicate potential problems and suggest solutions
Bachelorâs degree plus minimum 24 months experience; Masters Degree preferred
Basic laboratory skills and prior molecular biology laboratory experience is required.
A degree in Biochemistry, Cell Biology or related science field is preferred.
Previous research experience working with mice is preferred.
Required skills: strong analytical and communication skills; the ability to work independently; computer skills including word processing, data entry, statistical analysis, and graphic presentation.
Vanderbilt University Medical Center is a comprehensive health care facility dedicated to patient care, research, and biomedical education. Our reputation for excellence in these areas has made us a major center for patient referrals from throughout the Mid-South. Each year, people throughout Tennessee and the Southeast choose Vanderbilt University Medical Center for their health care because of our leadership in medical science and our dedication to treating patients with dignity and compassion.The mission of Vanderbilt University Medical Center is to advance health and wellness through preeminent programs in patient care, education, and research.